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MBL Life science clear back (human fc receptor blocking reagent
Clear Back (Human Fc Receptor Blocking Reagent, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/clear+back+(human+fc+receptor+blocking+reagent/human+fc+receptor+blocking+reagent/pm39027379-213-12-19
Average 90 stars, based on 1 article reviews
clear back (human fc receptor blocking reagent - by Bioz Stars, 2026-10
90/100 stars

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Incubation:

Article Title: Suppression of MIP-2 or IL-8 production by annexins A1 and A4 during coculturing of macrophages with late apoptotic human peripheral blood neutrophils.
Article Snippet: The HeLa cells or human neutrophils were centrifuged, and the cell pellet was incubatedwith FITC-labeled annexin V and propidium iodide (PI) for 5 min at r.t. After washingwith PBS, the cells were suspended in a sheath solution (Iso Flow) (Beckman Coulter) and filtered through a mesh, followed by flow cytometric analysis with FACSCalibur. .. The HeLa cells or human neutrophils were centrifuged and the cell pellet was incubated with human Fc receptor blocking reagent (Clear Back) (MBL, Nagoya) for 5 min at r.t. .. The cells were then incubated with 0.5 μg anti-human ANXA1 antibodies, anti-human ANXA4 antibodies, or control normal IgG (TOYOBO) for 30 min on ice, followed by washing with PBS containing 0.5% BSA and 0.01% NaN3 (staining buffer) twice and further incubation with 0.5 μg biotinylated goat anti-rabbit IgG F(ab′)2 fragment (American Qualex).

Article Title: Impact of mature dendritic cells pulsed with a novel WT1 helper peptide on the induction of HLA‑A2‑restricted WT1‑reactive CD8+ T cells.
Article Snippet: .. To assess DC phenotypes, imDCs or mDCs (1x105/100 μl) were washed, incubated with Clear Back human Fc receptor blocking reagent (dilution 1:20; cat. no. MTG‐001; MBL Life Science) for 5 min at 4 ̊C and then stained with the following monoclonal antibodies (mAbs) at 4 ̊C for 30 min: Fluorescein isothiocyanate (FITC)‐conjugated anti-human HLA-ABC (dilution 1:20; cat. no. 311404; clone W6/32), anti-CD80 (dilution 1:20; cat. no. 305206; clone 2D10), anti-CD40 (dilution 1:20; cat. no. 334306; clone 5C3; all from BioLegend, Inc.); FITC-conjugated anti-CD14 (dilution 1:20; cat. no. 11-0149-42; clone 61D3; eBioscience; Thermo .. anti-human HLA-DR (dilution 1:20; cat. no. 307606; clone L243), anti-CD11c (dilution 1:20; cat. no. 301606; clone 3.9), anti-CD83 (dilution 1:20; cat. no. 305308; clone HB15e) and anti-CD86 (dilution 1:20; cat. no. 305406; clone IT2.2; all from BioLegend, Inc.).

Blocking Assay:

Article Title: Suppression of MIP-2 or IL-8 production by annexins A1 and A4 during coculturing of macrophages with late apoptotic human peripheral blood neutrophils.
Article Snippet: The HeLa cells or human neutrophils were centrifuged, and the cell pellet was incubatedwith FITC-labeled annexin V and propidium iodide (PI) for 5 min at r.t. After washingwith PBS, the cells were suspended in a sheath solution (Iso Flow) (Beckman Coulter) and filtered through a mesh, followed by flow cytometric analysis with FACSCalibur. .. The HeLa cells or human neutrophils were centrifuged and the cell pellet was incubated with human Fc receptor blocking reagent (Clear Back) (MBL, Nagoya) for 5 min at r.t. .. The cells were then incubated with 0.5 μg anti-human ANXA1 antibodies, anti-human ANXA4 antibodies, or control normal IgG (TOYOBO) for 30 min on ice, followed by washing with PBS containing 0.5% BSA and 0.01% NaN3 (staining buffer) twice and further incubation with 0.5 μg biotinylated goat anti-rabbit IgG F(ab′)2 fragment (American Qualex).

Article Title: Impact of mature dendritic cells pulsed with a novel WT1 helper peptide on the induction of HLA‑A2‑restricted WT1‑reactive CD8+ T cells.
Article Snippet: .. To assess DC phenotypes, imDCs or mDCs (1x105/100 μl) were washed, incubated with Clear Back human Fc receptor blocking reagent (dilution 1:20; cat. no. MTG‐001; MBL Life Science) for 5 min at 4 ̊C and then stained with the following monoclonal antibodies (mAbs) at 4 ̊C for 30 min: Fluorescein isothiocyanate (FITC)‐conjugated anti-human HLA-ABC (dilution 1:20; cat. no. 311404; clone W6/32), anti-CD80 (dilution 1:20; cat. no. 305206; clone 2D10), anti-CD40 (dilution 1:20; cat. no. 334306; clone 5C3; all from BioLegend, Inc.); FITC-conjugated anti-CD14 (dilution 1:20; cat. no. 11-0149-42; clone 61D3; eBioscience; Thermo .. anti-human HLA-DR (dilution 1:20; cat. no. 307606; clone L243), anti-CD11c (dilution 1:20; cat. no. 301606; clone 3.9), anti-CD83 (dilution 1:20; cat. no. 305308; clone HB15e) and anti-CD86 (dilution 1:20; cat. no. 305406; clone IT2.2; all from BioLegend, Inc.).

other:

Article Title: A general pHLA-CD80 scaffold fusion protein to promote efficient antigen-specific T cell-based immunotherapy
Article Snippet: BV421 mouse anti-human CD4 (BD, 562424), PE-Cy7 mouse anti-human CD8 (BD, 557746), Clear Back (human Fc receptor blocking reagent) (MBL, MTG-001), and T-Select HLA-A∗02:01 CMV pp65 tetramer-NLVPMVATV-PE (MBL, TS-0010-1C) were used to identify tetramer-stained CD8 + T cells using flow cytometry.

Article Title: Immunological function of Langerhans cells in HIV infection.
Article Snippet: Background: Langerhans cells (LCs) are one of the initial target cells for HIV following sexual exposure and they are productively infected by HIV.. HIV-infected LCs migrate to the draining lymph nodes (dLNs) and transmit the virus to CD4 T cells, leading to the dissemination of HIV.. In contrast with the role of LCs in initial HIV acquisition, little is known about the modulation of immune responses by HIV-infected LCs.

Staining:

Article Title: Impact of mature dendritic cells pulsed with a novel WT1 helper peptide on the induction of HLA‑A2‑restricted WT1‑reactive CD8+ T cells.
Article Snippet: .. To assess DC phenotypes, imDCs or mDCs (1x105/100 μl) were washed, incubated with Clear Back human Fc receptor blocking reagent (dilution 1:20; cat. no. MTG‐001; MBL Life Science) for 5 min at 4 ̊C and then stained with the following monoclonal antibodies (mAbs) at 4 ̊C for 30 min: Fluorescein isothiocyanate (FITC)‐conjugated anti-human HLA-ABC (dilution 1:20; cat. no. 311404; clone W6/32), anti-CD80 (dilution 1:20; cat. no. 305206; clone 2D10), anti-CD40 (dilution 1:20; cat. no. 334306; clone 5C3; all from BioLegend, Inc.); FITC-conjugated anti-CD14 (dilution 1:20; cat. no. 11-0149-42; clone 61D3; eBioscience; Thermo .. anti-human HLA-DR (dilution 1:20; cat. no. 307606; clone L243), anti-CD11c (dilution 1:20; cat. no. 301606; clone 3.9), anti-CD83 (dilution 1:20; cat. no. 305308; clone HB15e) and anti-CD86 (dilution 1:20; cat. no. 305406; clone IT2.2; all from BioLegend, Inc.).

Bioprocessing:

Article Title: Impact of mature dendritic cells pulsed with a novel WT1 helper peptide on the induction of HLA‑A2‑restricted WT1‑reactive CD8+ T cells.
Article Snippet: .. To assess DC phenotypes, imDCs or mDCs (1x105/100 μl) were washed, incubated with Clear Back human Fc receptor blocking reagent (dilution 1:20; cat. no. MTG‐001; MBL Life Science) for 5 min at 4 ̊C and then stained with the following monoclonal antibodies (mAbs) at 4 ̊C for 30 min: Fluorescein isothiocyanate (FITC)‐conjugated anti-human HLA-ABC (dilution 1:20; cat. no. 311404; clone W6/32), anti-CD80 (dilution 1:20; cat. no. 305206; clone 2D10), anti-CD40 (dilution 1:20; cat. no. 334306; clone 5C3; all from BioLegend, Inc.); FITC-conjugated anti-CD14 (dilution 1:20; cat. no. 11-0149-42; clone 61D3; eBioscience; Thermo .. anti-human HLA-DR (dilution 1:20; cat. no. 307606; clone L243), anti-CD11c (dilution 1:20; cat. no. 301606; clone 3.9), anti-CD83 (dilution 1:20; cat. no. 305308; clone HB15e) and anti-CD86 (dilution 1:20; cat. no. 305406; clone IT2.2; all from BioLegend, Inc.).



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